Complementary methods for contact hypersensitivity (CHS) evaluation in mice

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• 作者：Magdalena Zemelka-Wi?cek^a ; Monika Majewska-Szczepanik^a ; Wies?aw Pyrczak^b ; Marian Szczepanik^a ; ^{mmszczep@cyf-kr.edu.pl}
• 关键词：ACD ; allergic contact dermatitis ; CHS ; contact hypersensitivity ; DDC ; dermal dendritic cells ; DNFB ; 1-fluoro-4 ; 6-dinitrobenzene ; DNP ; 2 ; 4-dinitrophenol ; DNP-BSA ; 2 ; 4-dintrophenylated bovine serum albumin ; ELNC ; auricular lymph node cells ; ELISA ; enzyme-li
• 刊名：Journal of Immunological Methods
• 出版年：2013
• 出版时间：31 January, 2013
• 年：2013
• 卷：387
• 期：1-2
• 页码：270-275
• 全文大小：380 K

文摘

Contact hypersensitivity (CHS) is an experimental model of allergic contact dermatitis (ACD) that can be studied in mice. For CHS responses, mice are immunized by painting with a reactive hapten, such as 1-fluoro-4,6-dinitrobenzene (DNFB), on the shaved abdominal and chest skin. Subsequently, the ears are challenged with diluted hapten, eliciting ¡®hypersensitive¡¯ ear-swelling reactions, which can be measured with a micrometer. In this manuscript we present complementary methods that can be used to evaluate CHS in mice that include: ear weight, vascular permeability, myeloperoxidase (MPO) activity, IFN-¦Ã concentration in ear extracts and also IFN-¦Ã production by auricular lymph node cells (ELNC). The biochemical evaluation of CHS can be also supported by proliferation assay, measurement of IFN-¦Ã production by skin-draining lymph node cells employing ELISA test and by evaluation of IFN-¦Ã⁺ TCR¦Á¦Â CD8 cells with the use of flow cytometry.