Rapid multi-residue analysis of antibiotics in muscle by liquid chromatography–tandem mass spectrometry
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- 作者：K. Granelli ; Christina Elgerud ; Å ; sa Lundströ ; m ; Anita Ohlsson ; Pernilla Sjö ; berg
- 关键词：Antibiotics ; Tetracyclines ; Sulfonamides ; Quinolones ; β ; -Lactams ; Macrolides ; Muscle ; Liquid chromatography– ; tandem mass spectrometry ; Veterinary drugs ; Multi-residue
- 刊名：Analytica Chimica Acta
- 出版年：2009
- 出版时间：1 April 2009
- 年：2009
- 卷：637
- 期：1-2
- 页码：87-91
- 全文大小：142 K
文摘
A way of carrying out simple and rapid multi-residue analysis of antibiotics in porcine and bovine muscle by liquid chromatography–tandem mass spectrometry (LC–MS/MS) is described. The method has previously been published as a screening method, but the scope has now been extended to quantification and confirmation.
Nineteen compounds from five different classes of antibiotics, i.e. tetracyclines, sulfonamides, quinolones, (-lactams and macrolides, are included in the method. The samples are extracted by a single extraction using 70 % methanol, diluted with water and injected in the LC–MS/MS. The run time is 7 min per injection. About 60 samples can be analysed in 24 h. By using this method the need for a separate screening step prior to confirmation is eliminated and consequently the total time from sampling to a confirmed result will be considerably reduced.
Validation was performed according to Commission Decision 2002/657/EC.