The Species at Risk (SPEAR) pesticide bioassessment index was adapted for a California watershed. Spatially and temporally explicit pesticide application data were linked to changes in stream invertebrate communities. Both peak and average exposure levels were important in determining community-level responses. The composition of macroinvertebrates trended toward taxa having higher resilience and resistance to pesticide exposure. Risk-assessment efforts can be improved by considering both the short- and long-term effects of pesticide mixtures.