Differential isoform expression and protein localization from alternatively spliced Apetala2 in peanut under drought stress

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• 作者：So-Yon Park ; ^{soypark@vt.edu" class="auth_mail" title="E-mail the corresponding author} ; Elizabeth Grabau
• 关键词：Apetala2 (AP2) ; Drought ; Peanut ; Alternative splicing
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 November 2016
• 年：2016
• 卷：206
• 期：Complete
• 页码：98-102
• 全文大小：1170 K

文摘

APETALA2 (AP2) belongs to the AP2/Ethylene Responsive Factor (ERF) family and regulates expression levels of downstream stress responsive genes as a transcription factor. In this study, we cloned six different isoforms of m>AhAP2m> from peanut (m>Arachis hypogaeam>). Four isoforms (AhAP2.1, AhAP2.2, AhAP2.3 and AhAP2.4) had both AP2/ERF DNA binding domains and ERF-associated amphiphilic repression (EAR) motifs. Two isoforms (AhAP2.5 and AhAP2.6) only had an EAR suppressor domain. After agroinfiltration, AhAP2.1, AhAP2.3, and AhAP2.4 fused to yellow fluorescent protein (YFP) showed localization to the nucleolus, which is the site of transcription and ribosome biogenesis. AhAP2.2-YFP showed a dispersed signal in the nucleus. AhAP2.5 and AhAP2.6 fused to YFP localized to both the nucleus and cytoplasm. In addition, increased levels of m>AhAP2.1m> and m>AhAP2.2m> transcripts were observed in drought-treated peanut leaves, suggesting differential transcriptional regulation under drought stress conditions.