Isolation of murine nickel-specific T cell hybridomas

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• 作者：Vulté ; e ; Christian V. ; Griem ; Peter ; Gleichmann ; Ernst
• 关键词：nickel allergy ; murine T cell hybridomas
• 刊名：Toxicology Letters
• 出版年：1996
• 出版时间：October, 1996
• 年：1996
• 卷：88
• 期：Complete
• 页码：75
• 全文大小：120 K

文摘

The molecular mechanism of allergies to heavy metals, such as nickel, is still elusive. This is the first report on the isolation of murine nickel-specific T cell hybridomas. Female C57BL/6 mice were immunized with nickel compounds plus mouse serum albumin (MSA) which is known to be a nickel-binding protein. MSA was preincubated with either Ni(II)Cl₂ or Ni(IV)(OH)₂O which exerts strong oxidizing effects on organic compounds. Neither Ni(II)Cl₂ nor Ni(IV)(OH)₂O led to the isolation of T cell hybridoma clones specific for nickel-altered MSA, i.e. the metal-protein complex used for priming. Following immunization with Ni(II)Cl₂ plus MSA, one clone was found that recognized splenic antigen-presenting cells (APC) without MSA in the presence of Ni(II), i.e. unknown nickel-altered self-protein. After immunization with Ni(IV)(OH)₂O-pretreated MSA, however, 61 clones recognizing APC plus Ni(II) were obtained, suggesting that a higher oxidation state of Ni is involved in T cell priming. For challenging of established clones though Ni(II) proved sufficient. Since the clones obtained did react to Ni(II) plus unknown self-proteins, but failed to react to the original nickel-MSA complexes, the role of MSA in T cell priming to nickel is obscure.