Evaluation of colorimetric detection methods for Shigella, Salmonella, and Vibrio cholerae by loop-mediated isothermal amplification
详细信息 查看全文
- 作者：Kevin W. Soli ; Monalisa Kas ; Tobias Maure ; Masahiro Umezaki ; Ayako Morita ; Peter M. Siba ; Andrew R. Greenhill ; Paul F. Horwood
- 关键词：LAMP ; Loop-mediated isothermal amplification ; Colorimetric detection ; Hydroxynaphthol blue ; SYBR Green ; Salmonella ; Shigella ; Vibrio cholerae
- 刊名：Diagnostic Microbiology and Infectious Disease
- 出版年：December, 2013
- 年：2013
- 卷：77
- 期：4
- 页码：321-323
- 全文大小：199 K
文摘
We evaluated loop-mediated isothermal amplification end-point detection methods for m>Salmonella m>, m>Shigella m>, and m>Vibrio cholerae m>. Detection sensitivities were comparable to real-time PCR methods. The colorimetric dyes hydroxynaphthol blue and SYBR Green I showed increased sensitivity when compared to visual and automated turbidity readings. End-point colorimetric dyes promise great utility in developing settings.