Determination of the oxido-redox status of plasma albumin in hemodialysis patients

详细信息    查看全文

• 作者：Maurizio Bruschi ; Andrea Petretto ; Giovanni C ; iano ; Luca Musante ; Ezio Movilli ; Laura Santucci ; Andrea Urbani ; Rosanna Gusmano ; Enrico Verrina ; Giovanni Cancarini ; Francesco Scolari ; Gian Marco Ghigge
• 关键词：Plasma albumin ; Oxidation stress ; Oxidation markers ; Hemodialysis
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2008
• 出版时间：15 March 2008
• 年：2008
• 卷：864
• 期：1-2
• 页码：29-37
• 全文大小：472 K

文摘

The oxido-redox status of plasma albumin in patients treated with hemodialysis was characterized with LC–ESI-MS/MS and was compared with models of oxidative stress. Oxidised albumin was characterized by sulfonation (SO₃⁻) of the SH at Cys 34, unfolding and acidification of the molecule. Albumin in hemodialysis patients presented, instead, only intermediate oxidation products such as sulfenic (SO₂), sulfonic (SO) and methionine sulfoxide (C₅H₉NO₂S) involving Cys 165–269 and Met 329–548 but did not present SO₃⁻ at Cys 34. Absence of charge and structural alterations compared to the oxidised templates was also confirmed with electrophoretic titration and calorimetry. In conclusion, the oxido-redox status of plasma albumin in hemodialysis patients lacks the hallmarks of the advanced oxidation products. LC–ESI-MS/MS was crucial to characterize albumin in conditions of oxidation stress; surrogate techniques can mirror conformational changes induced by oxidation.