Derivation of Trisomy 21 affected human embryonic stem cell line Genea021

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• 作者：Biljana Dumevska ; ¹ ; Alexis Bosman¹ ; Robert McKernan ; Heather Main ; Uli Schmidt ; Teija Peura
• 刊名：Stem Cell Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：16
• 期：2
• 页码：401-404
• 全文大小：622 K

文摘

The Genea021 human embryonic stem cell line was derived from a donated, fully commercially consented ART blastocyst, carrying Trisomy 21, indicative of Down Syndrome. Following ICM outgrowth on inactivated human feeders, CGH and STR analyses demonstrated a 47, XY, + 21 karyotype and male allele pattern. The hESC line had pluripotent cell morphology, 71% of cells expressed Nanog, 84% Oct4, 23% Tra1–60 and 95% SSEA4, gave a Pluritest Pluripotency score of 21.85, Novelty of 1.42, demonstrated Alkaline Phosphatase activity and tri-lineage teratoma formation. The cell line was negative for Mycoplasma and visible contamination.