A new expression vector facilitating production and functional analysis of scFv antibody fragments selected from Tomlinson I + J phagemid libraries
详细信息 查看全文
- 作者：Karolina Ossyseka ; karolina.ossysek@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Tomasz Ucha艅skia ; 1 ; tomasz.uchanski@vib-vub.be" class="auth_mail" title="E-mail the corresponding author ; Ma艂gorzata Kuleszaa ; malgorzata.kulesza@doctoral.uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Monika Bzowskaa ; b ; monika.bzowska@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Tomasz Klausa ; b ; tomasz.klaus@doctoral.uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Klaudia Wo艣a ; klaudia.wos@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Mariusz Madeja ; 2 ; mariusz.madej@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author ; Joanna Beretaa ; joanna.bereta@uj.edu.pl" class="auth_mail" title="E-mail the corresponding author
- 关键词：scFv ; Tomlinson I ; + ; J ; Expression vector ; Amber codon
- 刊名：Immunology Letters
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：167
- 期：2
- 页码：95-102
- 全文大小：1289 K
文摘
- •
A new pET-scFv-T vector is compatible with Tomlinson I + J scFv phagemid libraries.
- •
pET-scFv-T/Rosetta system ensures efficient expression of functional scFvs.
- •
pET-scFv-T circumvents the problem of in-frame amber codons in scFvs.