The profiling of the metabolites of hirsutine in rat by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap mass spectrometry: An improved strategy for the systematic screening and identification of metabolites in multi-samples in vivo

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• 作者：Jianwei Wang^a ; ^b ; ^c ; ¹ ; Peng Qi^b ; ¹ ; Jinjun Hou^b ; Yao Shen^b ; Min Yang^b ; Qirui Bi^a ; ^b ; Yanping Deng^b ; Xiaojian Shi^a ; ^b ; Ruihong Feng^b ; Zijin Feng^b ; Wanying Wu^b ; ^{wanyingwu@simm.ac.cn} ; Dean Guo^a ; ^b ; ^{daguo@simm.ac.cn}
• 关键词：Hirsutine ; Metabolic profile ; Multi-samples ; Metabolites systematic screening ; Uncaria rhynchophylla ; UHPLC/LTQ-Orbitrap-MS
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：149-157
• 全文大小：2837 K
• 卷排序：134

文摘

An integral strategy was developed for systemic screening and identifying of metabolites from multiple biological samples. The data processing method of combination of MMDF and tool Progenesis QI based on UPLC-LTQ-Orbitrap MS was applied. The workload of metabolites identification with the new approach decreased 46%. 67 metabolites of hirsutine in rat multiple biological samples were identified and an in-house library was built. Comprehensive metabolic profile of hirsutine was proposed for the first time.