Separation of enantiomers of chiral weak acids with polysaccharide-based chiral columns and aqueous-organic mobile phases in high-performance liquid chromatography: Typical reversed-phase behavior?

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• 作者：Iza Matarashvili^a ; Darejan Ghughunishvili^b ; Lali Chankvetadze^a ; Nino Takaishvili^a ; Tamar Khatiashvili^a ; Maia Tsintsadze^b ; Tivadar Farkas^c ; Bezhan Chankvetadze^a ; ^{jpba_bezhan@yahoo.com}
• 关键词：Enantioseparations ; Chiral HPLC ; Polysaccharide-based chiral stationary phases ; Reversal of enantiomer elution order
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：3 February 2017
• 年：2017
• 卷：1483
• 期：Complete
• 页码：86-92
• 全文大小：1918 K
• 卷排序：1483

文摘

HILIC-like behavior of polysaccharide-based chiral stationary phases under aqueous-organic mobile phase conditions was observed. Redistribution of polar- and hydrophobic forces involved in chiral recognition based on the content of water in the mobile phase may lead to a reversal of enantiomer elution order in HPLC. Very different effect of water and n-hexane additives on the retention and enantioselectivity on polysaccharide-based chiral stationary phases was observed.