Antimicrobial and cell viability measurement of bovine serum albumin capped silver nanoparticles (Ag/BSA) loaded collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film

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• 作者：Rotimi Bakare^a ; Samantha Hawthrone^a ; Carmen Vails^b ; Ayele Gugssa^b ; Alamgir Karim^c ; John Stubbs III^d ; Dharmaraj Raghavan^a ; ^{draghavan@howard.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Protein conjugated silver nanoparticles ; Collagen immobilized PHBV ; Antimicrobial activity ; Bone cell viability ; Atomic absorption spectrometry
• 刊名：Journal of Colloid and Interface Science
• 出版年：2016
• 出版时间：1 March 2016
• 年：2016
• 卷：465
• 期：Complete
• 页码：140-148
• 全文大小：1042 K

文摘

Bacterial infection of orthopedic devices has been a major concern in joint replacement procedures. Therefore, this study is aimed at formulating collagen immobilized poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film loaded with bovine serum albumin capped silver nanoparticles (Ag/BSA NPs) to inhibit bacterial growth while retaining/promoting osteoblast cells viability. The nanoparticles loaded collagen immobilized PHBV film was characterized for its composition by X-ray Photoelectron Spectroscopy and Anodic Stripping Voltammetry. The extent of loading of Ag/BSA NPs on collagen immobilized PHBV film was found to depend on the chemistry of the functionalized PHBV film and the concentration of Ag/BSA NPs solution used for loading nanoparticles. Our results showed that more Ag/BSA NPs were loaded on higher molecular weight collagen immobilized PHEMA-g-PHBV film. Maximum loading of Ag/BSA NPs on collagen immobilized PHBV film was observed when 16 ppm solution was used for adsorption studies. Colony forming unit and optical density measurements showed broad antimicrobial activity towards Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa at significantly lower concentration i.e., 0.19 and 0.31 μg/disc, compared to gentamicin and sulfamethoxazole trimethoprim while MTT assay showed that released nanoparticles from Ag/BSA NPs loaded collagen immobilized PHBV film has no impact on MCTC3-E1 cells viability.