Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China

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• 作者：Kai Wei Xu^a ; ¹ ; ^{xkwei@sicau.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Lan Zou^a ; ¹ ; Petri Penttinen^b ; ¹ ; Xiangzhong Zeng^c ; Ming Liu^a ; Ke Zhao^a ; Cuiping Chen^a ; Yuan Xue Chen^a ; ^{cyxue2002@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Xiaoping Zhang^a ; ^{zhangxiaopingphd@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Desmodium ; Rhizobia ; Housekeeping genes ; Genetic diversity ; MLSA
• 刊名：Systematic and Applied Microbiology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：39
• 期：1
• 页码：33-40
• 全文大小：564 K

文摘

Thirty-four rhizobial isolates were obtained from root nodules of four wild Desmodium species growing in Panxi, Sichuan, China. According to the combined ARDRA and IGS-RFLP (CACAI) cluster analysis, Rhizobium, Pararhizobium and Mesorhizobium isolates outnumbered Bradyrhizobium isolates. In general, the isolates representing the same species from the same site clustered together. Furthermore, the four Desmodium species were all nodulated by more than one rhizobial species. AFLP and phenotypic analyses showed that the 34 isolates represented at least 32 distinct strains. None of the strains were found from more than one site or host, indicating a high degree of rhizobial diversity in Panxi. In the multilocus sequence analysis, the isolates were assigned to Pararhizobium giardinii, Bradyrhizobium japonicum, Mesorhizobium septentrionale, and to undescribed species of the genera Rhizobium, Bradyrhizobium and Agrobacterium.