The anti-inflammatory potential of Cortex Phellodendron in vivo and in vitro: Down-regulation of NO and iNOS through suppression of NF-魏B and MAPK activation

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• 作者：You Yeon Choi^a ; Mi Hye Kim^a ; Jae Min Han^a ; Jongki Hong^b ; Tae-Hee Lee^c ; Sung-Hoon Kim^d ; Woong Mo Yang^a ; ^{wmyang@khu.ac.kr" class="auth_mail}
• 关键词：Phellodendron amurense ; Lipopolysaccharide (LPS) ; Cytokine ; Inflammation
• 刊名：International Immunopharmacology
• 出版年：April, 2014
• 年：2014
• 卷：19
• 期：2
• 页码：214-220
• 全文大小：988 K

文摘

Cortex Phellodendri amurensis (CPA), derived from the dried bark of Phellodendron amurense Rupr., is a traditional medicine widely used to treat various inflammation-related diseases. The aim of this study was to investigate the anti-inflammatory activity and molecular mechanism of CPA in vivo and in vitro.

Mice were pretreated with CPA (200 mg/kg, p.o.) for three consecutive days; 2 h after the last CPA treatment, mice were intraperitoneally injected with lipopolysaccharide (LPS) to induce endotoxemia (35 mg/kg). After treatment, we assessed survival rate, protein levels and cytokine expression. In addition, we confirmed the molecular mechanism of anti-inflammatory effects of CPA in LPS-stimulated macrophage RAW 264.7 cells.

The results showed that CPA significantly increased mice survival rates and down-regulated LPS-induced interleukin (IL)-6, IL-1尾 and macrophage chemo-attractant protein (MCP)-1 in serum. In addition, CPA inhibited inducible nitric oxide synthase (iNOS), activation of nuclear factor (NF)-魏B by degradation and phosphorylation of I魏B伪, and attenuated phosphorylation of mitogen-activated protein kinases (MAPKs; ERK 1/2, p38 and JNK) from mice challenged with LPS. Moreover, in RAW 264.7 cells, CPA dose-dependently down-regulated LPS-stimulated NO, iNOS expression, as well as inflammatory cytokines and protein expression, consistent with the results in vivo.

The anti-inflammatory properties of CPA in vitro and in vivo suggest its utility for attenuating inflammation-related diseases.