Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex

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• 作者：Qiong Hu^a ; Minhui He^a ; Yaqi Mei^a ; Wenjie Feng^b ; Su Jing^b ; Jinming Kong^a ; ^{j.kong@njust.edu.cn} ; Xueji Zhang^a ; ^c ; ^{xueji@usf.edu}
• 关键词：Colorimetric ; Alkaline phosphatase ; Bathophenanthroline disulfonate ; Cu(II)-phenanthroline complex ; Metal-to-ligand charge-transfer ; Naked-eye
• 刊名：Talanta
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：163
• 期：Complete
• 页码：146-152
• 全文大小：629 K
• 卷排序：163

文摘

We report a simple and practical method for the colorimetric assay of ALP activity. We exploit the Cu(BPDS)23− complex as the reporter for its distinctive MLCT properties. It allows a naked-eye readout of ALP activity and is highly immune to false positives. It is selective and holds great potential in ALP inhibitor efficiency evaluation. It is practical for its satisfactory analytical performance in complex serum samples.