B-lymphocytes forming follicle-like structures in the lung tissue of tuberculosis-infected mice: Dynamics, phenotypes and functional activity

详细信息    查看全文

• 作者：Irina Linge^a ; Alexander Dyatlov^a ; Elena Kondratieva^a ; Vadim Avdienko^a ; Alexander Apt^a ; ^b ; ¹ ; ^asapt@aha.ru ; Tatiana Kondratieva^a ; ¹
• 关键词：Tuberculosis ; B-lymphocytes ; Lung tissue ; Surface phenotype ; Monoclonal antibodies
• 刊名：Tuberculosis
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：102
• 期：Complete
• 页码：16-23
• 全文大小：1323 K
• 卷排序：102

文摘

During tuberculosis (TB) infection, B cells form follicles in close vicinity of lung granuloma. We assessed the dynamics of follicle formation, surface phenotypes and functional activity of lung B cells during TB course in genetically susceptible mice. The follicles appeared early post infection and peaked at weeks 7–8. Lung B cells resembled classical B2 cells (CD19+IgMloIgDhiCD1d−CD21/35intCD5−CD11b−CD43−), but differed from them by the absence of B2 marker CD23. Lung B-cells constitutively expressed MHC II molecules, presented mycobacterial antigens to immune CD4+ T-cells and produced high amounts of IL-6 and IL-11, but no classical type 1 (TNF-α, IFN-γ), or anti-inflammatory (IL-10, TGF-β) cytokines. The total antibody response in tuberculous lung showed almost no specificity to mycobacteria. A panel of monoclonal antibodies obtained from lung B cells contained only few clones with reactivity to mycobacteria. Our results suggest that anti-TB B cell response in the lung has clear pathological and doubtful protective role.