Giardia and Cryptosporidium spp. dissemination during wastewater treatment and comparative detection via immunofluorescence assay (IFA), nested polymerase chain reaction (nested PCR) and loop mediated isothermal amplification (LAMP)
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- 作者：Carmen Gallas-Lindemanna ; b ; 1 ; gallas-lindemann.c@lineg.de" class="auth_mail" title="E-mail the corresponding author ; gallaslindemann@gmx.de" class="auth_mail" title="E-mail the corresponding author ; Isaia Sotiriadoua ; c ; 1 ; Judit Plutzerd ; Michael J. Noackc ; Mohammad Reza Mahmoudie ; Panagiotis Karanisa ; f
- 关键词：IFA ; immunofluorescence assay ; nested PCR ; nested polymerase chain reaction ; LAMP ; loop-mediated isothermal amplification ; WWTP ; wastewater treatment plant ; DIC ; differential interference contrast ; DAPI ; diamino-2-phenylindole ; EF1A ; elongation factor 1α ; SSU rRNA ; small subunit of ribosomal RNA ; SAM-1 ; S-adenosyl-l-methionine synthetase-1
- 刊名：Acta Tropica
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：158
- 期：Complete
- 页码：43-51
- 全文大小：2162 K
文摘
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Comparative detection of Giardia and Cryptosporidium oocysts via IFA, PCR and LAMP.
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Monitoring of WWTPs, surface waters, a recreational area, groundwater and tap water.
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Indications that Giardia and Cryptosporidium are common in all water types.
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20">Molecular apps are sensitive in detecting target DNA but may not cover all genotypes.
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25">Molecular methods are effective for tracking the sources of contamination.