Family of hemorphins: co-relations between amino acid sequences and effects in cell cultures

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• 作者：Blishchenko ; Elena Y. ; Sazonova ; Olga V. ; Kalinina ; Olga A. ; Yatskin ; Oleg N. ; Philippova ; Marina M. ; et. al.
• 关键词：FBS ; fetal bovine serum ; GPI ; guinea pig ileum ; Hemorphin ; Proliferation ; Cytotoxicity
• 刊名：Peptides
• 出版年：2002
• 出版时间：May, 2002
• 年：2002
• 卷：23
• 期：5
• 页码：903-910
• 全文大小：118 K

文摘

Hemorphins, i.e. endogenous fragments of β-globin chain segment (32–41) LVVYPWTQRY(F) suppress the growth of transformed murine fibroblasts L929 cell culture, the effect is due to cytotoxicity and inhibition of cell proliferation. The contribution of cytotoxicity depends on the presence of Leu³²: VV-hemorphins, except VV-hemorphin-4, exhibit cytotoxicity significantly higher than respective LVV-hemorphins. Decrease of cell number induced by hemorphins depend on the extent of N- and C-terminal degradation of hemorphins: VV-hemorphins in most cases are more active than LVV-, V-hemorphins, and hemorphins. In the group of VV-hemorphins the activity of VV-hemorphin-5 (valorphin) is significantly higher than of VV-hemorphin-7, VV-hemorphin-6, and VV-hemorphin-4, meaning that the presence of C-terminal Gln is important for suppressing of cell number. The amino acid sequence VVYPWTQ corresponding to valorphin was identified as important for manifestation of the both cytotoxic and antiproliferative effects.