Effect of protein-tyrosine phosphatase 4A3 by small interfering RNA on the proliferation of lung cancer

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• 作者：Yi-Xin Lian^a ; Rui Chen^a ; Yue-Hua Xu^a ; ^{yuehua_xu@163.com} ; Chun-Lei Peng^b ; Hua-Cheng Hu^a
• 关键词：PTP4A3 ; protein-tyrosine phosphatase 4A3 ; PRLs ; phosphatases of regeneration liver ; MAPK ; mitogen activated protein kinase ; EMT ; epithelial-mesenchymal transition ; PTEN ; protein tyrosine phosphatase ; PI3K-AKT ; phosphatidylinositol-3-kinase ; QPCR ; quantita
• 刊名：Gene
• 出版年：2012
• 出版时间：15 December, 2012
• 年：2012
• 卷：511
• 期：2
• 页码：169-176
• 全文大小：1138 K

文摘

Previous studies on PTP4A3 mainly focused on tumor metastasis due to the close relationship between the overexpression of lung cancer and metastasis. However, the role of PTP4A3 in the proliferation of tumor still has remained unclear. To investigate the role of PTP4A3 in cell growth of lung cancer, we constructed PTP4A3-siRNA expressing lentivirus and infected human lung cancer H1299 cells, and then examined the inhibitory effect of PTP4A3 in vitro. The levels of PTP4A3 mRNA and protein in H1299 cells decreased after PTP4A3-siRNA lentivirus infection. The growth and colony formation of the infected cells were also inhibited, indicating that PTP4A3 gene is closely associated with the proliferation of H1299 cells. In addition, after PTP4A3 specific siRNA lentivirus infection, it was notable that whilst H1299 cells in G1 phase apparently reduced, both of H1299 cells in G2/M phase and the cell apoptosis increased significantly. This finding indicated the close relationship between PTP4A3 gene and apoptosis in the H1299 cells. These results come to their conclusion that PTP4A3 plays an important role in the growth of lung cancer cells. PTP4A3 may be considered as a valuable target for anti-tumor therapeutic strategies.