Fast-tumbling bicelles constructed from native Escherichia coli lipids
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- 作者：Jobst Liebaua ; Pontus Petterssona ; Philipp Zubera ; Candan Ariö ; za ; b ; Lena Mä ; lera ; lena.maler@dbb.su.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：CL ; cardiolipin ; DHPC-d22 ; tail-deuterated 1 ; 2-dihexanoyl-sn-glycero-3-phosphocholine ; DMPC ; 1 ; 2-dimyristoyl-sn-glycero-3-phosphocholine ; DMPG ; 1 ; 2-dimyristoyl-sn-glycero-3-phosphoglycerol ; NOE ; nuclear Overhauser enhancement ; PC ; phosphatidylcholine ; PG ; phosphatidylglycerol ; PE ; phosphatidylethanolamine ; TLC ; thin layer chromatography
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：1858
- 期：9
- 页码：2097-2105
- 全文大小：844 K
文摘
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Bicelles with native E. coli lipids can be produced.
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Bicelles lacking the major lipid PE can be made by using PE-deficient E. coli.
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Lipid composition can be fine-tuned by adjusting growth temperature and growth phase.
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The lipids in E. coli bicelles display complex dynamics on the ps–ns time-scale.