Reorganization of plasma membrane lipid domains during conidial germination

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• 关键词：Di-4-ANEPPS ; 4-(2-(6-(dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-pyridinium ; DOPC ; 1 ; 2-dioleoyl-sn-glycero-3-phosphocholine ; DPH ; 1 ; 6-diphenyl-1 ; 3 ; 5-hexatriene ; DPPC ; 1 ; 2-dipalmitoyl-sn- glycero-3-phosphocholine ; FGSC ; Fungal Genetics Stock Center ; IPC ; inositolphosphorylceramide ; MMV ; Vogel&rsquo ; s Minimal Medium ; PC ; phosphatidylcholine ; PE ; phosphatidylethanolamine ; PhyCer ; Phytoceramide ; POPC ; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ; SC ; Synthetic Complete Medium ; SeM
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1862
• 期：2
• 页码：156-166
• 全文大小：928 K
• 卷排序：1862

文摘

Conidia germination includes major reorganization and fluidization of the membrane. N. crassa conidia lack ergosterol, but contain sphingolipid (SL)-enriched domains. Those domains are gel-like, melting near growth temperature for N. crassa wild type. Cell wall-less mutant (slime) unable to form hyphae has more and tighter SL domains. Slime membrane biophysical properties resemble those of unicellular S. cerevisiae.