Selective membrane disruption by the cyclotide kalata B7: complex ions and essential functional groups in the phosphatidylethanolamine binding pocket
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- 作者：Adam A. Strö ; mstedta ; ; Per Eugen Kristiansenb ; Sunithi Gunasekeraa ; Nathalie Groba ; Lars Skjeldalc ; Ulf Gö ; ranssona
- 关键词：AMP ; antimicrobial peptide ; PE ; phosphatidylethanolamine ; PC ; phosphatidylcholine ; PG ; phosphatidylglycerol ; POPE/PC/PG ; 1-palmitoyl-2-oleoyl-sn-glycero-3-phospholipids ; DPC ; dodecylphosphocholine ; kB1&ndash ; 7 ; kalata B1-7 ; cyO2 ; cycloviolacin O2 ; TFA ; trifluoroacetic acid ; DMF ; dimethylformamide ; ACN ; acetonitrile
- 刊名：Biochimica et Biophysica Acta (BBA)-Biomembranes
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：1858
- 期：6
- 页码：1317-1327
- 全文大小：1784 K
文摘
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Presence of PE-lipids increased membrane disruption by kB7 up to 740-fold.
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10">Structural analogs mutated at Tyr15 showed contrasting PE-selectivity.
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15">High PE-selectivity of kB7 requires a phenyl group at position 15.
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20">The PE-binding pocket is co-localized with the divalent cation complex site.
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25">Presence of divalent cation complexes does not affect PE-affinity.