Interaction of Cecropin B with Zwitterionic and Negatively Charged Lipid Bilayers Immobilized at Gold Electrode Surface

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• 作者：Joanna Juhaniewicz^a ; ¹ ; Lilianna Szyk-Warszyńska^b ; Piotr Warszyński^b ; Sławomir Sęk^a ; ¹ ; ^{slasek@chem.uw.edu.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Supported lipid bilayers ; Bioelectrochemistry ; Atomic force microscopy ; Gold electrodes ; Antimicrobial peptides
• 刊名：Electrochimica Acta
• 出版年：2016
• 出版时间：20 June 2016
• 年：2016
• 卷：204
• 期：Complete
• 页码：206-217
• 全文大小：3973 K

文摘

Membranolytic properties of cationic antimicrobial peptide cecropin B were investigated using electrochemical techniques, atomic force microscopy and quartz crystal microbalance with dissipation monitoring. Two types of artificial lipid bilayers supported on gold electrode were used as model systems composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol (Chol) at 7:3 molar ratio and L-α-phosphatidylethanolamine (E. coli) (PE), L-α-phosphatidylglycerol sodium salt (E. coli) (PG) at 8:2 molar ratio. Thus the lipid content was intended to represent either mammalian or bacterial membrane respectively. Model bilayers were exposed to cecropin B at 1 μM concentration and the changes in bilayer structure, permeability and morphology were monitored as a function of time. We have found that cecropin B does not show any pronounced effect on POPC/Chol bilayer, while PE/PG system was strongly affected in the presence of the peptide. This observation suggests that cecropin B shows some selectivity with respect to lipid composition of the membrane. In case of PE/PG membrane, we have observed that peptide action involves electrostatically driven adsorption of the cecropin B at the top of the bilayer with simultaneous fluidization and swelling of the membrane. The latter may facilitate the rearrangement and insertion of the molecules into the core of the lipid bilayer, which leads to further rupture and degradation of the film through formation of mixed peptide-lipid aggregates.