Acute responses of DNA repair proteins and StarD6 in rat hippocampus after domoic acid-induced excitotoxicity

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• 作者：In Youb Chang^a ; Jin Ho Kim^b ; Kyung Won Cho^b ; Sang Pil Yoon^c ; ^{spyoon@jejunu.ac.kr}
• 关键词：APE ; apurinic/apyrimidinic endonuclease ; BER ; base excision repair ; CA ; Cornu ammonis ; DA ; domoic acid ; DNA-PK ; DNA-dependent protein kinase ; DNA-PKcs ; catalytic subunit of DNA-PK ; ¦Ã-H2AX ; phospho-histone H2AX ; NHEJ ; non-homologous end joining ; Ogg1 ; 8-o
• 刊名：Acta Histochemica
• 出版年：2013
• 出版时间：April, 2013
• 年：2013
• 卷：115
• 期：3
• 页码：234-239
• 全文大小：970 K

文摘

StarD6, which might be considered to be neuroprotective, and DNA repair proteins can play a role against oxidative damages by excitotoxin in the nervous system. In order to investigate the relationship between StarD6 and DNA repair proteins, excitotoxicity was induced by domoic acid in male Sprague-Dawley rats. Western blot analysis revealed transitorily elevated levels in StarD6, apurinic/apyrimidinic endonuclease (APE) and 8-oxoguanine DNA-glycosylase (Ogg1) in accord with the DNA damage marker phosphorylated H2AX. Immunohistochemistry revealed that increased intensity was transiently seen not only in the Stratum (Str.) radiatum and Str. lacunosum-moleculare with StarD6 and APE, but also in the Str. pyramidale with Ogg1. Intensities decreased 24 h after domoic acid injection in CA3 with APE and Ogg1 as well as in the Str. radiatum and Str. lacunosum-moleculare with StarD6 and APE. These results suggested that StarD6 may not be closely related with DNA repair proteins in the hippocampus after domoic acid-induced excitotoxicity, although the activities of these proteins might be positively affected by excitotoxic stimuli.