CO2 reduction and organic compounds production by photosynthetic bacteria with surface displayed carbonic anhydrase and inducible expression of phosphoenolpyruvate carboxylase
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- 作者：Ju-Yong Parka ; Yang-Hoon Kimb ; kyh@chungbuk.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Jiho Mina ; jihomin@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Surface displayed CA ; Carbonic anhydrase ; Phosphoenolpyruvate carboxylase ; CO2 reduction ; Organic compounds production
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：103-110
- 全文大小：1312 K
文摘
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Outer membrane protein can be used as an anchoring motif to display several type of CA with different sizes at the surface of R. sphaeroides.
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Inducible over-expression of CA and PEPC with surface displayed CA, two vectors systems, were developed.
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We have compared recombinant strains for the CO2 reduction and the production of the organic compounds.
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We have developed R. sphaeroides with enhanced photosynthetic activity through surface display of stable CA.