Up-regulation of hypoxia-inducible factor 1 alpha and hemodynamic responses following massive small bowel resection

详细信息    查看全文

• 作者：Kathryn J. Rowl ; Junjie Yao ; Lidai Wang ; Christopher R. Erwin ; Konstantin I. Maslov ; Lihong V. Wang ; Brad W. Warner
• 关键词：Small bowel resection (SBR) ; Intestine ; Hypoxia-inducible factor ; HIF1¦Á ; Oxygen saturation ; Blood flow ; Photoacoustic microscopy
• 刊名：Journal of Pediatric Surgery
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：48
• 期：6
• 页码：1330-1339
• 全文大小：968 K

文摘

Purpose

Massive small bowel resection (SBR) results in an adaptive response within the remnant bowel. We have previously shown an immediate reduction in intestinal blood flow and oxygen saturation (sO₂) after SBR. We therefore sought to determine the duration of resection-induced intestinal hypoxia and expression of hypoxia-inducible factors (HIFs) following SBR.

Methods

C57B6 mice were subjected to 50 % proximal SBR or a sham procedure. Photoacoustic microscopy (PAM) was used to measure blood flow and sO₂ on postoperative days (PODs) 1, 3, and 7. Ileal tissue was harvested 6 h postoperatively and on PODs 1 and 2, and HIF1¦Á, HIF2¦Á, and VEGF mRNA expression were assessed via RT-PCR. A p value of less than 0.05 was considered significant.

Results

Following SBR, reduction in intestinal blood flow persists for 24 h and is followed with hyperemia by POD 3. The immediate reduction in venous sO₂ and increased tissue oxygen utilization continued through POD 7. Enhanced expression of HIF1¦Á was demonstrated 6 h following SBR.

Conclusion

Massive SBR results in an immediate relative hypoxic state within the remnant bowel with early enhanced expression of HIF1¦Á. On POD 7, increased tissue oxygen extraction and elevated blood flow persist in the adapting intestine.