Fabricating antigen recognition and anti-bioadhesion polymeric surface via a photografting polymerization strategy
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- 作者：Lingjie Song ; Jie Zhao ; Jing Jin ; Jiao Ma ; Jingchuan Liu ; Shifang Luan ; Jinghua Yin
- 关键词：Photografting polymerization ; Anti-bioadhesion ; Antibody immobilization
- 刊名：Materials Science and Engineering: C
- 出版年：1 March, 2014
- 年：2014
- 卷：36
- 期：Complete
- 页码：57-64
- 全文大小：1648 K
文摘
A polymeric platform for immunodiagnostic bioassay was constructed based on biostable polymeric support and two functional monomers, low-fouling methacryloyloxyethyl phosphorylcholine (MPC) and acrylic acid (AAc), by a photografting polymerization approach. Covalent binding of antibody to pAAc chains was achieved by activating carboxyl with NHS/EDC partner. The resultant surface showed obvious suppression of nonspecific protein adsorption and platelet adhesion relative to the control sample, exhibiting good anti-bioadhesion performances. Based on the polymer-supported matrix, a highly sensitive antibody-antigen specific recognition was confirmed in both native plasma and diluted human plasma due to the enhanced antibody loading capacity and lowered bioadhesion as compared to the reference.