Photoactivated disinfection (PAD) of dental root canal system - An ex-vivo study

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• 作者：Dennis Mohan^a ; Sunith Maruthingal^a ; Rajamani Indira^b ; Darshan Devang Divakar^c ; ^{darshandevang@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Abdulaziz Abdullah Al Kheraif^c ; Ravikumar Ramakrishnaiah^c ; B.H. Durgesh^c ; Santhosh Basavarajappa^c ; Jacob John^d
• 关键词：Conventional endodontic treatment (CET) ; Colony forming unit (CFU) ; Chemo-mechanical preparation (CMP) ; Photo activated disinfection (PAD) ; Photosensitiser (PS) ; Tryptone Soya Broth (TSB) agar medium
• 刊名：Saudi Journal of Biological Sciences
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：23
• 期：1
• 页码：122-127
• 全文大小：390 K

文摘

To investigate the efficacy of photo activated disinfection (PAD) in reducing colony-forming unit (CFU) counts of Enterococcus faecalis (E. faecalis) in infected dental root canals. The study compared the efficacy of PAD with conventional endodontic treatment (CET) and also a combination of CET along with PAD.

Material and Methods

53 maxillary incisors were taken for the study. Teeth were divided into 3 groups, CET (Group I) (n = 11), PAD (Group II) (n = 21), and a combination of CET and PAD (Group III) which consisted of (n = 21) samples, Group II and Group III were further divided into 2 subgroups, Group IIa, IIb and Group IIIa, IIIb. Strains of E. faecalis were inoculated in all the root canals. CET group samples were treated by chemo-mechanical preparation (CMP) alone, PAD samples were treated with laser alone at 2 different exposure time (4 min and 2 min). In the combination treatment, samples were treated initially by CET and then by PAD for a time period of 4 min and 2 min. Contents of the root canal were aspirated, diluted and plated in Tryptone Soya Broth (TSB) and plates were incubated for 24 h to observe the bacterial regrowth.

Results

Showed PAD used along with CMP reduced the bacterial load of E. faecalis by 99.5% at 4 min and 98.89% at 2 min.

Conclusion

PAD may be an adjunctive procedure to kill residual bacteria in the dental root canal systems after standard endodontic root canal preparation.