An ultrarapid and regenerable microfluidic immunoassay coupled with integrated photosensors for point-of-use detection of ochratoxin A
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- 作者：R.R.G. Soaresa ; c ; 1Author Vitae ; Diogo Ramadasb ; 1Author Vitae ; V. ChuaAuthor Vitae ; M.R. Aires-BarroscAuthor Vitae ; J.P. Condea ; cAuthor Vitae ; A.S. VianadAuthor Vitae ; A.C. Cascalheirab ; accascalheira@fc.ul.pt" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Regenerable ; Rapid ; Microfluidic ; Immunoassay ; Photosensor ; Red wine
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：554-562
- 全文大小：2464 K
文摘
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A point-of-use microfluidic immunoassay for quantification of ochratoxin A was developed and optimized.
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The PDMS microfluidic chips were modularly coupled with an integrated optoelectronic detection system.
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Detection times of less than 5 min and sensitivities down to 0.5 ng/mL were obtained.
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The chips could be effectively regenerated at least 8 times.
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Ochratoxin A was measured in red wine extracts at the regulatory limits.