Nitrogen depletion in Arthrospira sp. PCC 8005, an ultrastructural point of view

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• 作者：Fré ; dé ; ric Deschoenmaeker^a ; ^{frederic.deschoenmaeker@umons.ac.be" class="auth_mail" title="E-mail the corresponding author} ; Raphaë ; l Facchini^a ; ^{raphael-facchini@outlook.com" class="auth_mail" title="E-mail the corresponding author} ; Juan Carlos Cabrera Pino^c ; ^{juan-carlos.cabrera@materianova.be" class="auth_mail" title="E-mail the corresponding author} ; Guillaume Bayon-Vicente^a ; ^{guillaume.bayon-vicente@student.umons.ac.be" class="auth_mail" title="E-mail the corresponding author} ; Neha Sachdeva^a ; ^{Neha.sachdeva@umons.ac.be" class="auth_mail" title="E-mail the corresponding author} ; Patrick Flammang^b ; ^{patrick.flammang@umons.ac.be" class="auth_mail" title="E-mail the corresponding author} ; Ruddy Wattiez^a ; ^{ruddy.wattiez@umons.ac.be" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CP ; cyanophycin ; EPS ; extracellular polymeric substance ; FT-IR ; Fourier transform-infrared spectroscopy ; HFN&minus ; high fluorescent nitrogen&minus ; cell ; PHA ; polyhydroxyalkanoate ; RPS ; released polysaccharide ; TGA ; thermogravimetric analysis
• 刊名：Journal of Structural Biology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：196
• 期：3
• 页码：385-393
• 全文大小：3037 K

文摘

In cyanobacteria, the nitrogen and carbon metabolisms are functionally bridged and consequently respond to the carbon-to-nitrogen ratio. Consequently, a nitrogen deficiency results in carbon excess. For the first time, the biological adaptation of Arthrospira sp. PCC 8005 to nitrogen starvation has been deeply characterized at the cellular structure scale. The results indicated that the carbon excess is rerouted into carbon storage granules, such as the polyhydroxyalkanoate and glycogen granules corroborating existing data. Additionally, this photosynthetic organism hugely secreted exopolysaccharides, which could constitute another biological carbon reservoir. It has been reported that few cells in trichomes of Arthrospira sp. PCC 8005 still display a high level of fluorescence after a long-term nitrogen starvation. The transmission electron microscopy showed that some cells still contained thylakoids and phycobilisomes after this long-term nitrogen starvation, which could explain the remaining fluorescence.