Arvanil and anandamide up-regulate CD36 expression in human peripheral blood mononuclear cells
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- 作者：Anna Maria Malfitano ; Gokce Altay Toruner ; Patrizia Gazzerro ; Chiara Laezza ; Seema Husain ; Davide Eletto ; Pierangelo Orl ; o ; Luciano De Petrocellis ; Alex ; ra Terskiy ; Marvin Schwalb ; et al.
- 关键词：Human peripheral blood mononuclear cells (PBMCs) ; Arvanil ; Anadamide ; RNA microarray ; CD36
- 刊名：Immunology Letters
- 出版年：2007
- 出版时间：15 April 2007
- 年：2007
- 卷：109
- 期：2
- 页码：145-154
- 全文大小：287 K
文摘
In this study we analysed the regulation of gene expression by arvanil and anandamide in human peripheral blood mononuclear cells (PBMCs) to clarify their immunosuppressive properties. PBMCs were activated, leading to CD36 down regulation, that was normalized by arvanil and anandamide. We used microarray technology to identify a regulatory pattern associated with cell proliferation in the presence of both substances. CD3–CD28 stimulated PBMCs showed a pattern of up-regulated and down-regulated genes after treatment with these substances. We selected and analysed several genes chosen by their function in the regulation of cell proliferation. We showed a transcriptional control of the CD36 gene by arvanil and anandamide associated with an increased protein expression, thus suggesting a possible role of CD36 in anandamide and arvanil anti-inflammatory pattern.