Species-specific identification of equine cyathostomes resistant to fenbendazole and susceptible to oxibendazole and moxidectin by macroarray probing
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- 作者：Donato Traversa ; Raffaella Iorio ; Domenico Otranto ; Annunziata Giangaspero ; Piermarino Milillo ; Thomas R. Klei
- 关键词：Horse ; Cyathostomes ; Drug resistance ; Benzimidazoles ; Oxibendazole ; Moxidectin ; Reverse Line Blot hybridization
- 刊名：Experimental Parasitology
- 出版年：2009
- 出版时间：January 2009
- 年：2009
- 卷：121
- 期：1
- 页码：92-95
- 全文大小：149 K
文摘
Cyathostome populations in horses on two farms located in central Italy with a history of fenbendazole (FBZ) resistance were investigated with the Faecal Egg Count Reduction Test to evaluate the susceptibility to oxibendazole and moxidectin. Faecal eggs were collected pre- and post-treatment on each farm and molecularly examined with a Reverse Line Blot (RLB) assay able to unequivocally detect and identify 13 cyathostome species. Resistance to FBZ was confirmed on both farms, while oxibendazole and moxidectin demonstrated 97 % and 100 % efficacy, respectively. Overall eight species of cyathostomes (Coronocyclus labiatus, Cylicocyclus ashworthi, Cylicocyclus nassatus, Cyathostomum catinatum, Cylicostephanus longibursatus, Cylicostephanus goldi, Cylicostephanus calicatus and Cylicocyclus insigne) were identified in pre-treatment samples. Coronocyclus labiatus and C. goldi were identified after treatment with FBZ while C. calicatus and C. labiatus were shown to be <100 % susceptible to oxibendazole. These data confirm that resistance to benzimidazoles is established in cyathostome populations from horse farms in Italy and that they are susceptible to moxidectin. The oxibendazole has been successfully demonstrated for the first time as effective against Italian populations of cyathostomes resistant to other benzimidazoles. The RLB assay herein used showed to be useful to study the distribution of these parasitic populations at species level under field conditions and could represent a powerful tool in broader investigation of drug resistance in horse farms from several countries.