Schwann cell autophagy counteracts the onset and chronification of neuropathic pain
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- 作者：Sara Marinelli ; Francesca Nazio ; Antonella Tinari ; Laura Ciarlo ; Marcello D鈥橝melio ; Luisa Pieroni ; Valentina Vacca ; Andrea Urbani ; Francesco Cecconi ; Walter Malorni ; Flaminia Pavone
- 关键词：Wallerian degeneration ; Glia ; Autophagic flux ; Chronic pain ; Behaviour ; Mice
- 刊名：Pain
- 出版年：January, 2014
- 年：2014
- 卷：155
- 期：1
- 页码：93-107
- 全文大小：5890 K
文摘
Axonal degeneration in peripheral nerves after injury is accompanied by myelin degradation initiated by Schwann cells (SCs). These cells activate autophagy, a ubiquitous cytoprotective process essential for degradation and recycling of cellular constituents. Concomitantly to nerve insult and axonal degeneration, neuropathic pain (NeP) arises. The role of SC autophagy in the mechanisms underlying NeP is still unknown. In this study, we examined the role of the autophagy during the early phase of Wallerian degeneration in NeP induction and chronification by using a murine model of peripheral nerve lesion (chronic constriction injury). We demonstrate that the autophagy inducer rapamycin, administered in the first week after nerve damage, induces long-lasting analgesic and antiinflammatory effects, facilitates nerve regeneration, and prevents pain chronification. Conversely, when autophagy is altered, by means of autophagic inhibitor 3-methyladenine administration or as occurs in activating molecule in Beclin-1-regulated autophagy transgenic mice (Ambra1+/gt), NeP is dramatically enhanced and prolonged. Immunohistochemical and ultrastructural evaluations show that rapamycin is able to increase autophagic flux in SCs, to accelerate myelin compaction, and to reduce inflammatory and immune reaction. Proteomic analysis combined with bioinformatic analysis suggests that a redox-sensitive mechanism could be responsible for SC autophagy activation. These data suggest that a deficiency of autophagic activity in SCs can be an early event in the origin of NeP chronification and that autophagy modulation may represent a powerful pharmacological approach to prevent the onset and chronification of NeP in the clinical setting.