Chromatographic profiling and identification of two new iridoid-indole alkaloids by UPLC-MS and HPLC-SPE-NMR analysis of an antimalarial extract from Nauclea pobeguinii

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• 作者：Yong-Jiang Xu^a ; Kenn Foubert^a ; Liene Dhooghe^a ; Filip Lemiè ; re^b ; Kanyanga Cimanga^a ; ^c ; Kahunu Mesia^c ; Sandra Apers^a ; Luc Pieters^a ; ^{luc.pieters@ua.ac.be}
• 关键词：Nauclea pobeguinii ; Rubiaceae ; Iridoid-indole alkaloids ; Naucleidinic acid ; 19-O-Methyl-3 ; 14-dihydroangustoline ; HPLC-SPE-NMR ; UPLC&ndash ; MS
• 刊名：Phytochemistry Letters
• 出版年：2012
• 出版时间：June, 2012
• 年：2012
• 卷：5
• 期：2
• 页码：316-319
• 全文大小：200 K

文摘

The total 80 % EtOH extract of stem bark of Nauclea pobeguinii (Rubiaceae), which is active against uncomplicated falciparum malaria as shown in previous clinical studies, was analysed by means of UPLC-MS and HPLC-SPE-NMR. Apart from the main constituent, strictosamide, a series of minor constituents was identified, including two new iridoid-indole alkaloids, i.e. naucleidinic acid and 19-O-methyl-3,14-dihydroangustoline, together with 8 known iridoid-indole alkaloids, i.e. naucleidinal, magniflorine, naucleofficine D, two diastereoisomers of 3,14-dihydroangustoline, strictosidine, desoxycordifoline, 3¦Á,5¦Á-tetrahydrodeoxycordifoline lactam, and a phenol glycoside 3,4,5-trimethoxyphenol ¦Â-d-apiofuranosyl-(1-6)-¦Â-d-glucopyranoside (kelampayoside A).