Microalgal cytometric analysis in the presence of endogenous autofluorescent pigments
详细信息 查看全文
- 作者：Veronika Dashkovaa ; veronika.dashkova@nu.edu.kz" class="auth_mail" title="E-mail the corresponding author ; Einat Segevb ; Dmitry Malashenkova ; c ; Roberto Kolterb ; Ivan Vorobjeva ; d ; Natasha S. Bartenevaa ; e ; natasha.barteneva@childrens.harvard.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Microalgae ; Autofluorescence ; Emiliania huxleyi ; Flow and imaging cytometry ; Spectral flow cytometry ; Viability
- 刊名：Algal Research
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：19
- 期：Complete
- 页码：370-380
- 全文大小：2107 K
文摘
- •
We applied multilaser and spectral flow cytometry for optimization of microalgal analysis in the presence of autofluorescence.
- •
Separating fluorescent dyes between different excitation lasers for microalgal staining was an effective approach.
- •
SYTOX Blue viability dye demonstrated minimal spectral overlap and good resolution.
- •
Spectral flow cytometry with virtual filtering is an advantageous approach for heterogeneous autofluorescence.