Differentiation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis by specific PCR amplification of the RPS0 intron

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• 作者：M. del Pilar Vercher^a ; José ; M. Garc&#xed ; a Mart&#xed ; nez^a ; Emilia Cantó ; n^c ; Javier Pemá ; n^c ; M. Micaela Gó ; mez Garc&#xed ; a^b ; Eulogio Valent&#xed ; n Gó ; mez^a ; Lucas del Castillo Agudo^a ; ^{Lucas.castillo@uv.es"" rel=""nofollow}
• 关键词：Candida ; Candida parapsilosis ; Candida orthopsilosis ; Candida metapsilosis ; Species identification ; PCR ; Intron ; RPS0
• 刊名：International Journal of Medical Microbiology
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：301
• 期：6
• 页码：531-535
• 全文大小：692 K

文摘

Although Candida parapsilosis is the most prevalent among the 3 species of the *psilosis group, studies applying DNA-based diagnostic techniques with isolates previously identified as C. parapsilosis have revealed that both C. orthopsilosis and C. metapsilosis account for 0–10 % of all these isolates, depending on the geographical area. Differences in the degrees of antifungal susceptibility and virulence have been found, so a more precise identification is required. In a first approach, we reidentified 38 randomly chosen clinical isolates, previously identified as C. parapsilosis, using the RPO2 (CA2) RAPD marker. Among them, we reclassified 4 as C. metapsilosis and 5 as C. orthopsilosis. We previously developed a method to identify different pathogen yeast species, including C. parapsilosis, based on the amplification of the RPS0 gene intron. In this work, we extend this approach to the new *psilosis species by partially sequencing their RPS0 gene, including the intron sequence. Based on intron sequences, we designed specific primers capable of identifying C. orthopsilosis and C. metapsilosis species, and we reidentified species among the initial isolates. These new primers have allowed a specific and rapid identification of C. orthopsilosis and C. metapsilosis.