An in vitro model to assess pneumococcal adherence to nasopharyngeal cells under competition conditions
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- 作者：Gowrisankar Rajam ; Delois Jackson ; Tamara Pilishvili ; Cynthia G. Whitney ; Richard R. Facklam ; George M. Carlone ; S ; ra Romero-Steiner
- 关键词：Competitive adherence ; In vitro adherence ; Nasopharyngeal cells ; Streptococcus pneumoniae
- 刊名：Journal of Microbiological Methods
- 出版年：2007
- 出版时间：August 2007
- 年：2007
- 卷：70
- 期：2
- 页码：219-226
- 全文大小：330 K
文摘
Pneumococcal conjugate vaccine (PCV7) reduces invasive disease and carriage caused by vaccine serotypes (VS). An increase in carriage and disease with non-vaccine serotypes (NVS) has been observed. We have developed an in vitro model with human nasopharyngeal (NP) epithelial cells (Detroit 562) to assess the adherence capacity of Streptococcus pneumoniae to NP cells in the presence or absence of a competing Pnc strain. Two hundred and fifty pneumococcal (Pnc) strains (10 strains per serotype for 7 VS and 18 NVS) were tested for their opacity phenotype. Strains exhibiting (≥ 50 % ) the transparent phenotype (n = 72) were evaluated for their adherence capacity to Detroit 562 cells. Mean adherence capacity (≥ 129 CFU/well) to NP cells was high for VS 18C, 4, and 9V and for NVS 16F, 10A, and 6A. In the in vitro competition experiments, VS strains out-competed (42/108) or co-existed (43/108) with NVS strains for adherence to NP cells in most co-inoculations. By contrast, NVS (15C, 16F, 31, and 35B) out-competed with VS in only 9 of 108 co-inoculations. Serotype 16F out-competed or co-existed with some VS and NVS strains. This model may be used to identify Pnc strains of a given serotype with competitive potentials for replacement of VS in the nasopharynx and to screen Pnc strains for animal colonization models.