Homo-trimeric Structure of the Type IVb Minor Pilin CofB Suggests Mechanism of CFA/III Pilus Assembly in Human Enterotoxigenic Escherichia coli
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- 作者：Kazuki Kawahara1 ; Hiroya Oki1 ; Shunsuke Fukakusa1 ; Takuya Yoshida1 ; Tomoya Imai2 ; Takahiro Maruno3 ; Yuji Kobayashi3 ; Daisuke Motooka4 ; Tetsuya Iida4 ; Tadayasu Ohkubo1 ; Shota Nakamura4 ; nshota@gen-info.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：ETEC ; type IV pili ; minor pilin ; pilus assembly ; protein crystallography
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 March 2016
- 年：2016
- 卷：428
- 期：6
- 页码：1209-1226
- 全文大小：3326 K
文摘
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In gram-negative bacteria, the question of how pilins are assembled to form a type IV pilus remains unknown.
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The type IV pilin CofB solely identified as a minor pilin in the colonization factor antigen/III pilus system promotes type IV pilus assembly.
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The CofB takes a homo-trimetric, tip-like structure, which is well situated at the top of the CFA/III pilus.
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This study enhances our understanding of the mechanism of filament assembly in type II secretion and type IV pilus systems.