Protective effect of n-butyl alcohol extracts from Rhizoma Pinelliae Pedatisectae against cerebral ischemia-reperfusion injury in rats

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• 作者：Yujie Ye^a ; ^b ; Juan Li^a ; Xiaolu Cao^b ; Yajun Chen^b ; Congjin Ye^a ; Keli Chen^a ; ^{kelichen@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Rhizoma Pinelliae Pedatisectae ; Cerebral ischemia-reperfusion injury ; Stroke ; Pretreatment
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：21 July 2016
• 年：2016
• 卷：188
• 期：Complete
• 页码：259-265
• 全文大小：1785 K

文摘

Rhizoma Pinelliae Pedatisectae (RPP) has been commonly used in traditional Chinese medicine (TCM) for treatment of various neurological related diseases. However, the mechanisms have not been fully clarified yet. The present study was designed to investigate the therapeutic effect of RPP against cerebral ischemic/reperfusion (I/R) injury in rats models, and more importantly, to explore the underlying mechanism.

Materials and methods

Cerebral ischemia and reperfusion was established through the classic middle cerebral artery occlusion (MCAO) for 2 h, followed by 24 h reperfusion. Rats were orally given different doses of n-butyl alcohol extracts (NBES) of RPP or saline for one week before induction of ischemia. Neurological defect scoring, cerebral infarct volume, oxidative stress markers, inflammatory reaction and nerve cell apoptosis were then estimated.

Results

It showed that NBES could alleviate in a dose-dependent manner neurological deficit and reduce the infarct volume in vivo. The protective effects of NBES were associated with increased Superoxide dismutase (SOD) activity and decreased malonaldehyde (MDA), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels. In addition, ischemia-induced neuron apoptosis was inhibited by NBES pretreatment, and western blot showed NBES upregulated expressions of B-cell leukemia-2 (Bcl-2) and downregulated Bcl-2-associated X (Bax) expression.

Conclusion

NBES prevent cerebral I/R injury by alleviating neuronal oxidative injury, inflammatory reaction and neuron apoptosis. The research for the traditional use of RPP provided certain theoretical basis.