Development of a strategy and process parameters for a green process in counter-current chromatography: Purification of tanshinone IIA and cryptotanshinone from Salvia miltiorrhiza Bunge as a case study

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• 作者：Min Zhang^a ; Svetlana Ignatova^b ; ^{svetlana.ignatova@brunel.ac.uk"" rel=""nofollow} ; Ping Hu^c ; ^{pinghu@ecust.edu.cn"" rel=""nofollow} ; Qionglin Liang^d ; Yiming Wang^d ; Guoan Luo^a ; ^d ; ^{luoga@tsinghua.edu.cn"" rel=""nofollow} ; Frank Wu Jun^e ; Ian Sutherland^b
• 关键词：High-performance counter-current chromatography ; Green purification process ; Tanshinone IIA ; Crypotanshinone ; Salvia miltiorrhiza Bunge
• 刊名：Journal of Chromatography A
• 出版年：2011
• 出版时间：9 September 2011
• 年：2011
• 卷：1218
• 期：36
• 页码：6031-6037
• 全文大小：493 K

文摘

A strategy for the development of a green process using counter-current chromatography technology is presented in this paper. The strategy began with solvent system selection, followed by linear scale-up from an analytical to a preparative process with optimized operating parameters. A two-stage separation using a multi-injection method was performed with a solvent system of hexane–dichloromethane–methanol–water (4:0.75:4:1) for the 1st stage and a hexane–ethanol–water (4:2:2) for the 2nd stage. A 191.8 mg of tanshinone IIA was purified, with a 97 % purity and 34.4 % recovery and a 276.7 mg of cryptotanshinone was separated, with a 95 % purity and 31.8 % recovery from 2.1 g of crude extract. Process parameters (throughput, efficiency, environmental risk factor and general process evaluation) and mass factors (mass intensity, separation mass efficiency and greenness) of a target were developed for monitoring of the counter-current chromatography process.