Complete ¹H-NMR and ¹³C-NMR spectral assignment of five malonyl ginsenosides from the fresh flower buds of Panax ginseng

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• 作者：Yu-Shuai Wang¹ ; Yin-Ping Jin¹ ; Wei Gao¹ ; Sheng-Yuan Xiao¹ ; ² ; Yu-Wei Zhang¹ ; Pei-He Zheng¹ ; Jia Wang¹ ; Jun-Xia Liu¹ ; Cheng-He Sun¹ ; Ying-Ping Wang¹ ; ^{yingpingw@126.net" class="auth_mail" title="E-mail the corresponding author}
• 关键词：flower buds ; ginsenoside ; malonyl ginsenoside Re ; NMR ; Panax ginseng
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：40
• 期：3
• 页码：245-250
• 全文大小：514 K

文摘

Ginsenosides are the major effective ingredients responsible for the pharmacological effects of ginseng. Malonyl ginsenosides are natural ginsenosides that contain a malonyl group attached to a glucose unit of the corresponding neutral ginsenosides.

Methods

Medium-pressure liquid chromatography and semipreparative high-performance liquid chromatography were used to isolate purified compounds and their structures determined by extensive one-dimensional- and two-dimensional nuclear magnetic resonance (NMR) experiments.

Results

A new saponin, namely malonyl-ginsenoside Re, was isolated from the fresh flower buds of Panax ginseng, along with malonyl-ginsenosides Rb1, Rb2, Rc, Rd. Some assignments for previously published ¹H- and ¹³C-NMR spectra were found to be inaccurate.

Conclusion

This study reports the complete NMR assignment of malonyl-ginsenoside Re, Rb₁, Rb₂, Rc, and Rd for the first time.