- 作者：Feng-Ying Liu ; Xue-Feng Wang ; Ming-Wei Li ; Jin-Mei Li ; Zhi-Qin Xi ; Guo-Ming Luan ; Jian-Guo Zhang ; Yu-Ping Wang ; Ji-Jun Sun ; Yun-Lin Li
- 关键词：Epilepsy ; Postsynaptic density-93 ; cDNA micro-array ; N-Methyl-d-aspartate receptors subunit 2B
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 出版时间：6 July 2007
- 年：2007
- 卷：358
- 期：3
- 页码：825-830
- 全文大小：658 K
To investigate the expression of PSD-93 mRNA and NR2B mRNA in the brain tissue from the patients with epilepsy so as to explore the possible mechanisms of the pathogenesis of the epilepsy.
Methods
Fifty-six patients with epilepsy were divided into intractable epilepsy (IE) and non-intractable epilepsy (NIE) groups. cDNA microarrays prepared from the brain tissues obtained from these two groups were scanned and comparison to those from the non-epileptogenic control (C) was made. Expression level of PSD-93mRNA and NR2BmRNA were examined by reverse transcription polymerase chain reaction (GAPDH gene, internal control). Expression ratio (target gene/GAPDH) was used to evaluate each gene relative expression level.
Results
The cDNA microarray analysis showed that the expression of PSD-93 mRNA related to the function of NMDAR-NO signal transduction pathway was significantly higher in epilepsy patients than those in the controlled group. The results of RT-PCR were consistent with those of the cDNA microarrays. The relative expression ratio of PSD-93 in patients with non-epileptogenic control, NIE, and IE was 0.159, 0.368, and 0.341, respectively. Correspondingly, that of NR2B was 0.198, 0.738, and 0.903, respectively. The expressions of PSD-93 and NR2B in the NIE and IE were significantly higher than those of control, respectively (P < 0.05). However, there was no significantly difference the expression of PSD-93 between NIE and IE. (P > 0.05), neither do that of NR2B (P > 0.05).
Conclusions
The upregulated expressions of PSD-93 mRNA and NR2BmRNA may be involved in the pathogenesis of epilepsy.