Dexamethasone treatment attenuates early seawater instillation-induced acute lung injury in rabbits
详细信息 查看全文
- 作者：Ding Xinmin ; Duan Yunyou ; Peng Chaosheng ; Feng Huasong ; Zhou pingkun ; Meng Jiguang ; Xue Zhiqian and Xu Qinzhi
- 关键词：Acute lung injury ; Seawater instillation ; Dexamethasone ; Alveolar fluid clearance ; Lung epithelial– ; endothelial barrier ; Lung ; Inflammation
- 刊名：Pharmacological Research
- 出版年：2006
- 出版时间：April 2006
- 年：2006
- 卷：53
- 期：4
- 页码：372-379
- 全文大小：389 K
文摘
There is very little evidence on the value of giving corticoids in cases of seawater drowning induced acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Therefore, this study aimed to investigate whether dexamethasone treatment can attenuate seawater instillation-induced acute lung injury in rabbits. Seawater (4 ml/kg body weight) was instilled into the lower trachea of ventilated, anesthetized rabbits. Then these rabbits were assigned randomly 20 min later to receive intravenous injection of 1 mg/kg body weight of dexamethasone (dissolving in 2 ml of normal saline) or 2 ml of normal saline. All animals demonstrated immediate drops in arterial oxygen tension (PaO2) and the total thoracic compliance, which were significantly improved after 2 h of dexamethasone treatment. Histopathological study also indicated that dexamethasone treatment markedly attenuated lung histopathological changes, alveolar hemorrhage and inflammatory cells infiltration with evidence of decreasing of myeloperoxidase (MPO) activity and tumor necrosis factor-α (TNF-α) concentration in lung tissue. In addition, dexamethasone treatment reduced extravascular lung water and lung epithelial–endothelial barrier permeability, up-regulated the expression of surfactant protein-A (SP-A) and α-epithelial Na+ channel, and increased Na+/K+-adenosine triphosphatase (Na+/K+-ATPase) activity and Na+/K+-ATPase-α1 protein abundance. Thus, these data indicate that dexamethasone treatment might be of benefit in patients with seawater aspiration-induced ALI.