DNA fragmentation and nuclear degradation during harvest-induced senescence of asparagus spears
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- 作者：Eason ; Jocelyn R. ; Pinkney ; Tatyana T. ; Johnston ; Jason W.
- 关键词：Asparagus ; Postharvest senescence ; DNA laddering ; PCD ; DAPI ; 4 ; 6-diamidino-2-phenylindole ; dsDNA ; double stranded DNA ; PCD ; programmed cell death ; TUNEL ; TdT-mediated dUTP nick end labelling
- 刊名：Postharvest Biology and Technology
- 出版年：2002
- 出版时间：September 1, 2002
- 年：2002
- 卷：26
- 期：2
- 页码：231-235
- 全文大小：124 K
文摘
DNA processing is a relatively early event during harvest-induced senescence of Asparagus officinalis spears. Nuclear DNA nicking and the fragmentation of genomic DNA into ladders was visualised by in situ TdT-mediated dUTP nick end labelling (TUNEL), gel electrophoresis and Southern analysis. Laddering was not visible at 1 h after harvest but was apparent after 6 h coinciding with increased senescence-associated RNA activity (Plant Physiol. 100 (1992) 1661), and preceding the physiological changes associated with asparagus spear senescence (Physiol. Plant. 80 (1990) 393).