11 Fetomaternal and peripheral immune status in preeclamptic and normotensive oocyte donation cases: Immune and inflammatory mechanisms

详细信息    查看全文

• 作者：Shigeru Saito^a ; Yasushi Nakabayashi^a ; Akitoshi Nakashima^b ; ^c ; Tomoko Adachi^d ; Masao Nakabayashi^d
• 刊名：Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：6
• 期：3
• 页码：142
• 全文大小：34 K

文摘

Oocyte donation (OD) is a high risk for preeclampsia because a fetus is a complete allograft to the maternal host. However the differences of immune status between preeclamptic OD cases and normotensive OD cases have not been clarified.

Objective

To clarify the immunological differences between preeclamptic OD cases and normotensive OD cases.

Methods

Deciduel CD3+Tcells, CD4+Tcells, CD8+Tcells, Foxp3+Treg cells CD56+NK cells and CD68+ macrophage were evaluated by immunohistochemistry.

Peripheral blood effector Treg cells were evaluated by flow cytometry in 5 preeclamptic OD cases and 16 normotensive OD cases.

Results

The populations of CD3+ T cells, CD4+ T cells, CD8+ T cells, Foxp3+ Treg cells, CD56+ NK cells and CD68+ macrophage in placental bed biopsy samples of OD cases were significantly lower compare to those in normal pregnancy subjects. Those levels in preeclamptic OD cases were similar to those in normotensive OD cases. Same findings were observed in preeclamptic cases in natural conception. Vascular remodeling of spiral arteries was very poor in OD cases regardless of the presence of preeclampsia. These findings were quite similar to those in preeclampsia of natural conception. Peripheral blood-effector Treg cells was significantly decreased in preeclampsia, but these levels in OD pregnancies were not changed in preeclamptic OD cases and normotensive OD cases.

Conclusion

Poor vascular remodeling and some of the immunological changes were observed in uncomplicated OD pregnancy, but we have not yet identified the crucial factors that lead to preeclampsia in OD pregnancy.