Quantitative analysis of post-translational modifications in human serum transthyretin associated with familial amyloidotic polyneuropathy by targeted LC-MS and intact protein MS
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- 作者：Marta Vilà ; -Ricoa ; Nú ; ria Colomé ; -Callsb ; Luna Martí ; n-Castelb ; Marina Gayc ; Sebastiá ; n Azorí ; nd ; Marta Vilasecac ; Antoni Planasa ; antoni.planas@iqs.edu" class="auth_mail" title="E-mail the corresponding author ; Francesc Canalsb ; fcanals@vhio.net" class="auth_mail" title="E-mail the corresponding author
- 关键词：Transthyretin ; Absolute quantification ; Post-translational modifications ; Intact protein analysis ; Targeted proteomics ; High resolution XIC quantification
- 刊名：Journal of Proteomics
- 出版年：2015
- 出版时间：8 September 2015
- 年：2015
- 卷：127
- 期：part_PB
- 页码：234-246
- 全文大小：735 K
文摘
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Setup of two complementary MS methods for the analysis of wt and V30M plasma TTR
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Quantification of wt:V30M TTR ratio and quantification of Cys-10 PTM isoforms by HR-XIC LC–MS
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Relative quantification of Cys-10 PTM TTR isoforms by intact protein analysis
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Robust methods for the characterization of Cys-10 PTM isoforms of amyloidotic TTR