Optimization of fermentative hydrogen production from hydrolysate of microwave assisted sulfuric acid pretreated oil palm trunk by hot spring enriched culture

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• 作者：Sontaya  ; Khamtib^a ; Pensri  ; Plangklang^a ; Alissara  ; Reungsang^a ;   ; ^b ;   ; ^{alissara@kku.ac.th}
• 关键词：Thermophilic ; Bio-hydrogen ; Oil palm trunk ; Microwave-H₂SO₄ pretreatment ; Response surface methodology ; Hydrolysate
• 刊名：International Journal of Hydrogen Energy
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：36
• 期：21
• 页码：14204-14216
• 全文大小：993 K

文摘

The optimum condition for hydrolysis of oil palm trunk (OPT) by microwave-H₂SO₄ method and hydrogen production from OPT hydrolysate were explored using response surface methodology (RSM) with central composite design (CCD). The optimum condition for hydrolysis of OPT was 1.56 % (w/v) H₂SO₄ and 7.50?min reaction time at 450?W. At this condition, 8.95¡À0.99?g/L glucose/L, 8.29¡À0.43?g/L xylose, 4.57¡À0.14?g/L arabinose and 2.61¡À0.11?g/L acetic were obtained. The individual and interactive effects of the initial pH, initial substrate concentration and NaHCO₃ concentration on the hydrogen production potential (P_s) from OPT hydrolysate by hot spring enriched culture were further investigated using RSM with CCD. The initial pH and initial substrate concentration had a statistically individual effect on P_s, while the interactive effect on P_s was found between the initial pH and initial substrate concentration. The optimum condition obtained was an initial pH of 6.03, an initial substrate concentration of 22.07?g/L and a NaHCO₃ concentration of 6.71?g/L in which a P_s of 1947?mL H₂/L medium was achieved. Microbial community analysis by PCR-DGGE indicated that Clostridium sp. and Lactobacillus sp. were the predominant species at the optimum condition.