Non-sterile bio-hydrogen fermentation from food waste in a continuous stirred tank reactor (CSTR): Performance and population analysis

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• 作者：Alissara Reungsang ; Chakkrit Sreela-or ; Pensri Plangklang
• 关键词：Bio-hydrogen ; Food waste ; CSTR
• 刊名：International Journal of Hydrogen Energy
• 出版年：22 November, 2013
• 年：2013
• 卷：38
• 期：35
• 页码：15630-15637
• 全文大小：871 K

文摘

Bio-hydrogen production from food waste by anaerobic mixed cultures was conducted in a continuous stirred tank reactor (CSTR). The hydraulic retention time (HRT) was optimized in order to maximize hydrogen yield (HY) and hydrogen production rate (HPR). The maximum hydrogen content (38.6%), HPR (379聽mL聽H₂/L.聽d) and HY (261聽mL聽H₂/g-VS_added) were achieved at the optimum HRT of 60聽h. The major soluble metabolite products were butyric and acetic acids which indicated a butyrate-acetate type fermentation. Operation of CSTR at HRT 60聽h could select hydrogen producing bacteria and eliminate lactic acid bacteria and acetogenic bacteria. The microbial community analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) revealed that the predominant hydrogen producer was Clostridium sp.