Fast detection of Southern tomato virus by one-step transcription loop-mediated isothermal amplification (RT-LAMP)

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• 作者：L. Elvira-Gonzá ; lez^a ; ¹ ; A.V. Puchades^a ; ¹ ; C. Carpino^a ; ^b ; A. Alfaro-Fernandez^c ; M.I. Font-San-Ambrosio^c ; L. Rubio^a ; ^d ; L Galipienso^a ; ^d ; ^e ; ^{galipienso_lui@gva.es}
• 关键词：STV ; Amalgamaviru ; Plant virus ; Solanum lycopersicum ; Seed-borne virus
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：241
• 期：Complete
• 页码：11-14
• 全文大小：1460 K
• 卷排序：241

文摘

A sensitive method for STV detection based on RT-LAMP is proposed. RT-LAMP is an isothermal method for nucleic acid amplification. STV can be detected from non-processed sap extracts of infected tomato plants.