Miscibility and interactions of animal and plant sterols with choline plasmalogen in binary and multicomponent model systems
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- 作者：Katarzyna H膮c-Wydro ; hac@chemia.uj.edu.pl" class="auth_mail ; Katarzyna Luty
- 关键词：Cancer cell membranes ; Choline plasmalogen ; Langmuir monolayers ; Phytosterols
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：1 April, 2014
- 年：2014
- 卷：116
- 期：Complete
- 页码：138-146
- 全文大小：1853 K
文摘
In this work miscibility and interactions of sterols with choline plasmalogen (PC-plasm) in Langmuir monolayers were studied. Moreover, the properties of cholesterol/phosphatidylcholine/plasmalogen mixtures of different PC-plasm concentration were investigated. The foregoing systems were treated as a model of cancer cell membranes, which are of higher plasmalogen level than normal cells. Finally, the influence of 尾-sitosterol and stigmasterol (phytosterols differing in anticancer potency) on these mixtures was verified. The properties of monolayers were analyzed based on the parameters derived from the surface pressure-area isotherms and images taken with Brewster Angle Microscope. It was found that at 30% of sterol in sterol/plasmalogen monolayer the lipids are immiscible and 3D crystallites are formed within the film. Cholesterol molecules mix favorably with PC-plasm at Xchol 鈮?#xA0;0.5, while the investigated phytosterols only at their prevailing proportion in binary system. The increase of choline plasmalogen in cholesterol/phosphatidylcholine monolayer causes destabilization of the system. Moreover, the incorporation of phytosterols into cholesterol/phosphatidylcholine + PC-plasm mixtures disturbed membrane morphology and this effect was stronger for 尾-sitosterol as compared to stigmasterol. It was concluded that the presence of vinyl ether bond at sn-1 position in PC-plasm molecule strongly affects miscibility of choline plasmalogen with sterols. The comparison of the collected data with those reported in literature allowed one to conclude that miscibility and interactions of sterols with PC-plasm are less favorable than those with phosphatidylcholine. It was also suggested that overexpression of plasmalogens in cancer cell membranes may be a factor differentiating sensitivity of cells to anticancer effect of phytosterols.